Cell viability data assessed by MTT test in rat tenocytes treated with sodium valproate

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Mota, Maria Clara Cruz; Oliveira, Camila Borges Martins de; dos Anjos, Elí Heber Martins; Mello, Maria Luiza Silveira, 2026, "Cell viability data assessed by MTT test in rat tenocytes treated with sodium valproate", https://doi.org/10.25824/REDU/HEAPSN, Repositório de Dados de Pesquisa da Unicamp , V1, UNF:6:LRIH807b/Xc0SSSg3C4AEg== [fileUNF]
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Description
The dataset identifies MTT results for rat tenocytes treated with sodium valproate (VPA). Among the various targets of the VPA action, there are indications that this drug may alter the macromolecular structure of collagen bundles in rat tendons. Since this effect may be a consequence of changes in gene expression in tenocytes, our laboratory is studying the effect of VPA on these cells. As an initial step in this investigation, we seek to select the ideal conditions of concentration and treatment time with the drug, during which the cells remain predominantly viable. For this purpose, primary cultures of tenocytes from passages #12 to #17, obtained from cells that migrated from tendon explants cultured for 8 days, were subjected to the MTT test after VPA treatment. The cell concentration was 2.0 x 105 cells mL-1, and the culture medium used was DMEM with high glucose, supplemented with fetal bovine serum, penicillin-streptomycin, and sodium pyruvate at 1%. The culture was maintained at 37 °C in a 5% CO2 atmosphere. Samples were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dissolved in dimethyl sulfoxide (DMSO, 200 μL) for the solubilization of the formed formazan crystals. Subsequently, agitation occurred for 20 min, followed by absorbance measurement using a microtiter plate reader at wavelengths of 560 nm and 690 nm. Controls were represented by cells cultured in the absence of the drug and were standardized as 100% viability. (2026-09-02)
Subject Medicine, Health and Life Sciences
Keyword Cell culture, MTT test, Tendon explant, Tenocyte, Valproic acid
Related Publication References: dos Anjos, E.H.M., Mello, M.L.S., Vidal, B.C. High-performance polarization microscopy reveals structural remodeling in rat calcaneal tendons cultivated in vitro. Cells 12, 566, 2023.doi: 10.3390/cells12040566
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Data on viability of rat tenocytes treated with sodium valproate.tab
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Published Nov 3, 2026
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2 Variables, 5 Observations UNF:6:LRIH807b/Xc0SSSg3C4AEg==
Data on viability of VPA-treated tenocytes
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