Technical feasibility of using supercritical CO₂ as a reaction medium for the sphingobium yanoikuyae biotransformation of R-(+)-limonene into R-(+)-α-terpineol

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Moreira, Rafael Chelala; Melo, Rayanne Priscilla França de; Martínez, Julian; Fasolin, Luiz Henrique; Bicas, Juliano Lemos, 2025, "Technical feasibility of using supercritical CO₂ as a reaction medium for the sphingobium yanoikuyae biotransformation of R-(+)-limonene into R-(+)-α-terpineol", https://doi.org/10.25824/redu/5FKDHN, Repositório de Dados de Pesquisa da Unicamp , V1

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Description	This chapter explores the use of supercritical carbon dioxide (SC-CO₂) as an alternative reaction medium in a biotransformation process mediated by a microbial enzyme. The study focuses on evaluating the technical feasibility of employing SC-CO₂ in a system involving the microbial conversion of a monoterpene compound into a value-added oxygenated derivative. In addition to assessing key reaction parameters, the work introduces a novel methodology for monitoring pH under supercritical conditions, contributing to the understanding of enzymatic inhibition phenomena in high-pressure systems. Although this approach led to methodological advances, the overall assessment indicates limitations in the applicability of SC-CO₂ for this specific biocatalytic process. The data cannot be made available for intellectual property protection reasons. For more information, contact the deposit managers.
Subject	Agricultural Sciences; Engineering
Keyword	Biotransformation, Supercritical CO₂, Enzyme inhibition, Monoterpene, Microbial catalysis, Pressure, Process evaluation, Biotechnology
Notes	The data cannot be made available for intellectual property protection reasons. For more information, contact the deposit managers.
License/Data Use Agreement	CC BY-NC 4.0


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Persistent Identifier	doi:10.25824/redu/5FKDHN
Publication Date	2025-05-28
Title	Technical feasibility of using supercritical CO₂ as a reaction medium for the sphingobium yanoikuyae biotransformation of R-(+)-limonene into R-(+)-α-terpineol
Author	Moreira, Rafael Chelala (Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos) - ORCID: https://orcid.org/0000-0001-9954-5868 Melo, Rayanne Priscilla França de (Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos) - ORCID: https://orcid.org/0000-0003-2632-4532 Martínez, Julian (Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos) - ORCID: https://orcid.org/0000-0002-8182-7207 Fasolin, Luiz Henrique (Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos) - ORCID: https://orcid.org/0000-0002-2398-0940 Bicas, Juliano Lemos (Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos) - ORCID: https://orcid.org/0000-0002-5252-4004
Point of Contact	Use email button above to contact. Bicas, Juliano Lemos (Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos) Moreira, Rafael Chelala (Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos)
Description	This chapter explores the use of supercritical carbon dioxide (SC-CO₂) as an alternative reaction medium in a biotransformation process mediated by a microbial enzyme. The study focuses on evaluating the technical feasibility of employing SC-CO₂ in a system involving the microbial conversion of a monoterpene compound into a value-added oxygenated derivative. In addition to assessing key reaction parameters, the work introduces a novel methodology for monitoring pH under supercritical conditions, contributing to the understanding of enzymatic inhibition phenomena in high-pressure systems. Although this approach led to methodological advances, the overall assessment indicates limitations in the applicability of SC-CO₂ for this specific biocatalytic process. The data cannot be made available for intellectual property protection reasons. For more information, contact the deposit managers.
Subject	Agricultural Sciences; Engineering
Keyword	Biotransformation Supercritical CO₂ Enzyme inhibition Monoterpene Microbial catalysis Pressure Process evaluation Biotechnology
Notes	The data cannot be made available for intellectual property protection reasons. For more information, contact the deposit managers.
Funding Information	Fundação de Amparo à Pesquisa do Estado de São Paulo: FAPESP: 2020/06814-3 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior: CAPES: 88887.470149/2019-00
Depositor	Chelala Moreira, Rafael
Deposit Date	2025-04-23
Declarações obrigatórias sobre ética e privacidade	o projeto que gerou os dados foi aprovado pelo Comite de Ética em Pesquisa da Unicamp ou não envolve questões que requeiram tal aprovação; os dados que serão depositados estão de acordo com a LGPD (Lei Geral de Proteção de Dados)

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