Markers for mitotic catastrophe in sodium valproate-treated HeLa cells

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Sforça, Beatriz Pierre; Mello, Maria Luiza Silveira, 2026, "Markers for mitotic catastrophe in sodium valproate-treated HeLa cells", https://doi.org/10.25824/redu/8OEBZ7, Repositório de Dados de Pesquisa da Unicamp , V1, UNF:6:m8UAxOJ1Ci8GhliXFj0R1A== [fileUNF]
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Description
The data set shows features for immunofluorescence signals which identify caspase-2 and p53 in HeLa cells cultured in the presence of 1 mM and 20 mM sodium valproate (VPA) for 4 h, and 20 mM VPA for 48 h. These proteins are ascribed to be potentially associated with the incidence of mitotic catastrophe, an oncosupressive event. Cells were fixed in methanol at -20 °C for 10 min, rinsed in PBS, permeabilized with 0.2% Triton X-100 for 10 min, and blocked in 5% BSA for 1 h. Rabbit anti-caspase-2 and anti-p53 were used as primary antibodies (1:200 and 1:100 dilutions, respectively). Goat anti-rabbit conjugated with Alexa-Fluor 488 (1:500 dilution) was used as secondary antibody. The images were captured in a Leica TCS SP5 II confocal microscope at the Laboratório Central de Tecnologias de Alto Desempenho em Ciências da Vida (LaCTAD/UNICAMP). The evaluated features were: total fluorescence area, mean gray value (average pixel intensity within a region of interest), standard deviation, minimum and maximal intensity (lowest and highest pixel intensity value within a region, respectively), integrated density (backgound-normalized sum of the pixel intensity values within the image or selection), and raw integrated density (sum of the values of the image pixels). (2025-11-17)
Subject Medicine, Health and Life Sciences
Keyword Imunofluorescência, HeLa cells, Sodium valproate, Mitotic catastrophe, Caspase-2, P53
Related Publication Felisbino MB, Tamashiro WMSC, Mello MLS. Chromatin remodeling, cell proliferation and cell death in valproic acid-treated HeLa cells. PLoS ONE 6: e29144, 2011.
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Parameters for caspase-2 fluorescence signals in VPA-treated HeLa cells.tab
Tabular Data - 29.5 KB
Published Jan 23, 2026
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26 Variables, 999 Observations UNF:6:Xp2YozrZr4lmx3mGmuXiNg==
Parameters for caspase-2 fluorescence signals in VPA-treated HeLa cells
Parameters for p53 fluorescence signals in VPA -treated HeLa cells.tab
Tabular Data - 29.5 KB
Published Jan 23, 2026
1 Download
26 Variables, 999 Observations UNF:6:Xp2YozrZr4lmx3mGmuXiNg==
Parameters for p53 fluorescence signals in VPA -treated HeLa cells
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