Replication data for: MicroRNAS e polimorfismos em receptor de vitamina D e interferons como possíveis biomarcadores de doença induzida elo SARS-CoV-2

Version 1.0
Moriel, Patricia, 2024, "Replication data for: MicroRNAS e polimorfismos em receptor de vitamina D e interferons como possíveis biomarcadores de doença induzida elo SARS-CoV-2", https://doi.org/10.25824/redu/WWUNT1, Repositório de Dados de Pesquisa da Unicamp, V1
Learn about Data Citation Standards.
Contact Owner Share
Dataset Metrics
17 Downloads
Description
O novo coronavírus (SARS-CoV-2), agente causador da Doença Coronavírus 2019 (COVID-19), se tornou a mais recente emergência mundial. Sabe-se que o SARS-CoV-2 entra nas células da mucosa respiratória através do receptor Enzima Conversora de Angiotensina 2 (ECA2), que é altamente expresso nas células epiteliais do pulmão, onde o vírus é capaz de provocar um grande processo inflamatório, caracterizando a síndrome respiratória aguda grave. Neste momento, buscamos utilizar nossa expertise nas áreas de genética e epigenética para contribuir de forma significativa no contexto da pandemia do SARS-CoV-2. Até janeiro de 2021, apenas seis artigos foram conduzidos em humanos para identificar possíveis microRNAs como biomarcadores de diagnóstico ou de gravidade da COVID-19 em amostras humanas e até o momento não existem trabalhos de validação destes ou de outros microRNAs (miRNAs) como biomarcadores da COVID-19. Assim, os objetivos deste projeto são: 1) Avaliar miRNAs como possíveis biomarcadores de diagnóstico da COVID-19 e 2) Identificar através de sequenciamento os principais miRNAs plasmáticos e polimorfismos no receptor de vitaina D e interferon relacionados à gravidade induzidas pela COVID-19. Para atingir o objetivo 1, será realizado um estudo observacional, analítico, transversal, cuja amostragem é não probabilística do tipo consecutiva. Para identificar miRNAs que possam ser biomarcadores de diagnóstico da COVID-19 (estudo transversal), serão formados dois grupos, grupo Caso (50 pacientes): amostra de sujeitos do Hospital Estadual de Sumaré (HES) que tiveram seu exame para SARS-CoV-2 positivo no teste referência [Reação em Cadeia da Polimerase em Tempo Real (RT-PCR)]; e grupo Controle (50 pacientes): amostra de voluntários que tiveram seu exame negativo para SARS-CoV-2 no teste referência (RT-PCR). Os miRNAs serão extraídos de plasma, utilizando kit comercial, de 6 amostras do grupo Caso e 6 amostras do grupo Controle para realização da montagem da biblioteca, sequenciamento e análise diferencial dos miRNAs expressos. Após análise, será realizada análise in sílico e serão estabelecidos os miRNAs mais promissores para validação. A validação dos miRNAs selecionados será realizada nas demais amostras. 2) Para atingir o objetivo 2, será realizado um estudo observacional, analítico, coorte retrospectivo, cuja amostragem é não probabilística do tipo consecutiva. Para identificar miRNAs e polimorfismos que possam ser biomarcadores de gravidade da COVID-19 (coorte retrospectivo), as amostras do grupo Caso (200 pacientes) serão estratificadas pela gravidade da doença (doença leve-moderada e doença grave-crítica) através de parâmetros clínicos e tomografia computadorizada de pulmão. Os miRNAs serão extraídos de 6 amostras de plasma dos pacientes que apresentaram doença leve-moderada e de 6 amostras dos pacientes que apresentaram doença grave-crítica, para posterior montagem da biblioteca, sequenciamento e análise diferencial. Após análise, será realizada análise in sílico e serão estabelecidos os miRNAs mais promissores para validação. A validação dos miRNAs selecionados será realizada nas demais amostras. A (2023-08-24)
Subject Medicine, Health and Life Sciences
Keyword miRNA, COVID-19, Polymorphism, Vitamin D
License/Data Use Agreement

CC BY-NC 4.0

1 File
planilha estatistica (1).xlsx
MS Excel Spreadsheet - 312.0 KB
Published Jun 24, 2024
17 Downloads
MD5: 3955ffe5c847cd9938a94dc12d0d1d01
Dados do estudo
Edit File

This file has already been deleted (or replaced) in the current version. It may not be edited.

Restrict Access

Restricting limits access to published files. People who want to use the restricted files can request access by default. If you disable request access, you must add information about access to the Terms of Access field.

Learn about restricting files and dataset access in the User Guide.

Enable access request
You must enable request access or add terms of access to restrict file access.
Save Changes
Edit Embargo

The selected file or files have already been published. Contact an administrator to change the embargo date or reason of the file or files.
Delete Files

The file will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Select File(s)

Please select one or more files.

Share Dataset

Share this dataset on your favorite social media networks.

Dataset Citations

Citations for this dataset are retrieved from Crossref via DataCite using Make Data Count standards. For more information about dataset metrics, please refer to the User Guide.

Sorry, no citations were found.
Restricted Files Selected

The selected file(s) may not be downloaded because you have not been granted access.

Download Options

The files selected are too large to download as a ZIP.

You can select individual files that are below the 953.7 MB download limit from the files table, or use the Data Access API for programmatic access to the files.

Select File(s)

Please select a file or files to be downloaded.

Restricted Files Selected

The restricted file(s) selected may not be downloaded because you have not been granted access.

Click Continue to download the files you have access to download.

Delete Dataset

Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.

Delete Draft Version

Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.

Unpublished Dataset Private URL

Private URL can only be used with unpublished versions of datasets.

Unpublished Dataset Private URL

Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your unpublished dataset.

Delete Files

The file(s) will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Compute

This dataset contains restricted files you may not compute on because you have not been granted access.

Deaccession Dataset

Are you sure you want to deaccession? The selected version(s) will no longer be viewable by the public.

Deaccession Dataset

Are you sure you want to deaccession this dataset? It will no longer be viewable by the public.

Version Differences Details

Please select two versions to view the differences.

Version Differences Details
 
Version:
Last Updated:
Version:
Last Updated:
Select File(s)

Please select a file or files for access request.

Select File(s)

Embargoed files cannot be accessed. Please select an unembargoed file or files for your access request.

Edit Tags

Select existing file tags or create new tags to describe your files. Each file can have more than one tag.

Request Access

  You need to Log In to request access.

Dataset Terms

This dataset is made available under the following terms. Please confirm and/or complete the information needed below in order to continue.

Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation shown on the dataset page.

CC BY-NC 4.0

Preview Guestbook

Upon downloading files the guestbook asks for the following information.

Account Information

Package File Download

Use the Download URL in a Wget command or a download manager to download this package file. Download via web browser is not recommended. User Guide - Downloading a Dataverse Package via URL

https://redu.unicamp.br/api/access/datafile/

Request Access

Please confirm and/or complete the information needed below in order to request access to files in this dataset.

Compute Batch
Clear Batch
Dataset Persistent Identifier Change Compute Batch
Compute Batch
Submit for Review

You will not be able to make changes to this dataset while it is in review.

Publish Dataset

Are you sure you want to republish this dataset?

Select if this is a minor or major version update.

Publish Dataset

This dataset cannot be published until Biomédicas is published by its administrator.

Publish Dataset

This dataset cannot be published until Biomédicas and Repositório de Dados de Pesquisa da Unicamp are published.

Return to Author

Return this dataset to contributor for modification.